Figure 6. INT-777 mediated improvement of glucose tolerance is TGR5-dependent.
A. TGR5+/+ and TGR5−/− male mice were fed a HF diet for 9 weeks and a first OGTT was performed thereafter. HF was then supplemented with INT-777 at 30mg/kg/d. A second OGTT was performed 4 weeks after treatment with INT-777 was initiated. Curves represent glucose tolerance before and after 4 weeks treatment with INT-777 in TGR5+/+ (left panel) and TGR5−/− (right panel) mice. The inset represents the average AUC. In TGR5+/+ mice, body weight before and after INT-777 treatment was 46.86±3.54g and 43.50±3.47g, respectively (n=8; not statistically different). In TGR5−/− mice, body weight before and after INT-777 treatment was 54.34±2.23g and 52.30±2.72g, respectively (n=8; not statistically different). B. Plasma insulin levels were concurrently measured during the OGTT in DIO in TGR5+/+ (left panel) and TGR5−/− (right panel) mice before and after 4 weeks treatment with INT-777. The inset represents the average AUC (n=8). The data are represented as mean±SE. Student’s unpaired t-test. * (P < 0.05) Vehicle compared to INT-777 treated mice