Figure 3.
Increased throughput of BBB experiments can be achieved by using a multiple spot sonication approach with the positioning system. A conventional approach to sonicating multiple locations is shown in (a) where each location is sonicated at a repetition frequency of 1 Hz after an initial injection (i) of microbubbles, and then the same process is repeated for each point (leaving approximately 5 min in between to enable the bubbles to clear the system). The modified approach in (b) uses the capability of the positioning system to translate to six locations per second to interleave the exposures such that each location still gets sonicated at a repetition frequency of 1 Hz. This approach can result in a significant reduction in time, as well as avoids multiple injections into small animals such as mice.