Figure 1.
Blood glucose (A), insulin (B), and NEFA (C) from hyperinsulinemic (10 mU · kg−1 · min−1)-euglycemic (∼8.0 mmol · liter−1) clamps in conscious, 5-h fasted ob/+ and ob/ob mice (n = 11–13 in each group). Ob/ob mice were sc injected with vehicle or FGF21 (1 mg · kg−1 · d−1) for 8 d, and jugular vein catheters were implanted 5 d before study. Vehicle-treated ob/+ were studied as an insulin-sensitive control. Blood samples were taken at indicated time points from the cut tail to measure blood glucose (D). The GIR (E) was adjusted as needed to maintain euglycemia. Mice were euthanized using a bolus of sodium pentobarbital at t = 120 min, and tissues were dissected and quickly frozen. All mice were male and studied at 9–10 wk of age. Data are presented as mean ± se, and the steady-state period was defined as t = 80–120 min during the clamp. Statistical significance was established at P < 0.05. *, Comparison to ob/+ mice; †, comparison between ob/ob mice treated with vehicle or FGF21; #, comparison within a group between basal and clamp conditions.