Figure 2.
Characterization of 143B, C8T and C9T cells. (A) Oxygen consumption measurements; endogenous respiration was measured in intact cells and after treatment with ATP synthase inhibitor oligomycin and uncoupler FCCP. (B) The total cellular ATP contents were measured in 1 × 106 cells with a luciferase detection kit. The oligomycin-resistant (Oligo-R) ATP contents were determined after cells were incubated with oligomycin for 20 min at 37°C at a concentration of 15 µg/ml. The oligomycin sensitive (Oligo-S) ATP production was calculated by subtracting the oligomycin-resistant portion from the total ATP content. Four measurements were carried out, and the error bars indicate two times of the standard errors of the mean. (C) Extracellular lactate level was determined in the media from 1 × 106 cells and calculated after 48 h of culturing. The error bars indicate the standard errors of the mean (*P < 0.05; **P < 0.01).