FIGURE 7. Interaction of SF-1 with CtBP1 helical bend mutants.
A, mammalian two-hybrid SF-1/CtBP interaction with WT CtBP1 or mutants near the helical bend were measured. The plasmids were transfected as in Fig. 6, the experiments were performed in triplicate, and the results are displayed with S.E. *, differs significantly from basal SF-1/WT CtBP1 signal. B, CYP17 −300 reporter (125 ng) activity was measured in the presence of SF-1 (25 ng), and WT or mutant CtBP1 or homologous site mutants of CtBP2 (25 ng). Symbols indicate significant difference from basal (^) or Bt2cAMP-stimulated (‡)SF-1-induced reporter activity in the absence of over- expressed CtBP.