Fig. 3. Simultaneous Na+ and Ca2+ imaging in GFP+ cartwheel cells.
(A) Maximum intensity projection of two GFP+ cartwheel cells. Red arrowheads trace axons.
(B) Top: somatic current drives 12 simple spikes at 100 Hz. Bottom: example of simultaneous Ca2+ (X-rhod-5F) and Na+ (CoroNa) transients 17 μm from axon hillock. Decay was fit with single exponential (black).
(C) Na+ (CoroNa, open circles) and Ca2+ (X-rhod-5F, closed circles) transients in soma (black), axon (red) and dendrites (orange). Bars are SEM.
(D) Na+ transient amplitude vs. Ca2+ transient amplitude at each imaging location in axon. n = 39 sites from 4 cells.
