Table 2.
mRNA expression levels of fibulin-1 and fibulin-2 in hepatic stellate cells (HSC), hepatocytes (HC), and myofibroblasts (MF) related to the expression level in control rat liver (in fold difference) by real-time RT-PCR (TGFβ1 transforming growth factor beta-1). Beta-actin was used as an endogeneous control. The expression levels in TGF-β1-treated cells were compared with control cells by Student’s t-test
| Samples | Fibulin-1, fold difference | P-value (t-test) | Fibulin-2, fold difference | P-value (t-test) | |
|---|---|---|---|---|---|
| HSC, day 7 | Control | 122.65±22.36 | - | 3.10±0.61 | - |
| 1 ng/ml TGFβ1 | 138.95±33.39 | 0.243 | 13.12±8.94 | 0.316 | |
| HC | Control | 2.71±0.91 | - | 0.12±0.09 | - |
| 1 ng/ml TGFβ1 | 2.37±0.54 | 0.280 | 0.52±0.34* | 0.027 | |
| MF, passage 2 | Control | 469.48±57.28 | - | 1517.99±24.25 | - |
| 1 ng/ml TGFβ1 | 463.65±52.79 | 0.462 | 3988±898.04* | 0.027 | |
*Significant changes