Figure 5.
The bistratified diving ganglion cell is distinguished by arbors in both sublamina a (cyan) and b (red) and, most diagnostically, the return of the processes in sublamina a to sublamina b before their final termination (arrows). Some processes have their complete arbor in sublamina b, but many other processes in the periphery of the dendritic field appear disconnected at that level of focus. The disconnected processes in sublamina b are connected to the dendritic arbor in sublamina a at the point they descend back to sublamina b (arrows). Inset, Rotation of optical sections into the z-axis shows how sharply dendrites in sublamina a descend back to sublamina b (arrow). The arrowhead marks the termination of a dendrite wholly stratified in sublamina b. The green bands are cholinergic processes stained with anti-ChAT for reference. The box indicates the area sampled in Figure 6. Scale bars: 100 μm; inset, 10 μm. All optical sections are 0.3 μm thick. The main panel is a stack of 70 × 0.3 μm optical sections; the inset is a stack of 6 × 0.3 μm optical sections.