Figure 1. Cortisol-induced nuclear translocation of GR slowed in primary human neuronal cultures with preincubation with 10 μM FK506.
Primary human neuroglial cultures were pretreated with vehicle (1:500 ethanol) or 10 μM FK506 for 2 hr and subsequently treated with 100 nM Cortisol for 0 (a), 60 (b), 180 (c) min. The cell cultures were immunolabeled for neuronal markers (MAP2 and NF; green), and GR (red), and nuclei counterstained with Hoechst (blue); arrows indicate GR localization. More focal nuclear staining of GR was apparent in a time-dependent manner with cortisol. Increased cytoplasmic GR is noted at 180 min in cultures pretreated with FK506, as shown by fluorescence intensity plots; arrows indicate nuclear GR (d).