Figure 4.
Leucine-rich repeat kinase-2 (LRRK2) expression or aggregate formation does not change levels of GFP-u. LRRK2-V5 was used to transfect cells that stably express a short-lived GFP that is degraded by the proteasome (GFP-u). Double immunofluorescence was performed between a monoclonal anti-V5 antibody (red) and GFP (green). Under basal conditions (A), cells with aggregates (arrows) do not increase GFP fluorescence. (B) MG132 treatment (10 µM, 16 hours) increased GFP fluorescence of all cells. GFP colocalized with LRRK2 aggregates in some cells (arrowhead). Images are representative of 3 independent experiments. Bar scale: 50 µm.