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. 2009 Jul 6;119(8):2317–2329. doi: 10.1172/JCI37378

Figure 4. CD4+ T cells from Ccr6–/– mice are able to differentiate into Th17/IL-22–producing cells.

Figure 4

(A) Naive CD4+ T cells (CD62L+CD44loCD25–) were purified from WT or Ccr6–/– mice and activated and cultured under nonpolarizing (NP) or Th17-polarizing conditions. After 7 days, cells were treated with PMA and ionomycin, and mRNAs for Il17a, Il22, and Gapdh were measured by real-time RT-PCR. Fold changes were calculated for Il17a and Il22 mRNAs normalized for Gapdh mRNA versus the nonpolarized sample with the greatest ΔCt within each plot. Values denote cells from WT or Ccr6–/– mice from a single representative experiment of 4. (B) Splenocytes were cultured in medium alone or with 100 ng/ml IL-23 for 12 hours, and mRNAs for Il22 and Gapdh were measured by real-time RT-PCR. Fold changes were calculated for values of Il22 mRNA normalized for Gapdh mRNA versus WT cells in medium alone. Data are from 3 samples from WT or Ccr6–/– mice in 1 representative experiment of 2. *P < 0.05 versus medium. Values for IL-23–treated splenocytes from WT and Ccr6–/– mice were not significantly different.