Figure 4. Effects of Clinically-Observed JM Mutations on EGFR Activity.
A. The noted clinically-observed JM mutations were introduced into the Flag-tagged EGFR ICD. The resulting constructs were transiently expressed in Cos-7 cells, and ICD autophosphorylation was assessed as described in Figure 1. B. and C. The V665M, L668P, and P670L mutations were also introduced into full-length EGFR, and the influence on EGF-dependent (+) and EGF-independent (−) EGFR autophosphorylation was assessed as described for Figure 1C. The K721R inactivating mutation was included as a negative control (C).