FIGURE 7.
Isolated islets from ATGL−/− mice show reduced insulin release in response to glucose, palmitate, and KCl. A, insulin secretion in islets isolated from overnight fasted male ATGL−/− or ATGL+/+ mice incubated for 1 h in KRBH with 0.5% d-BSA at 2.8, 8.3, or 16.7 mm glucose (G) in the presence or absence of 0.4 mm palmitate (Pal) and at 2.8 mm glucose plus 35 mm KCl. Cont, control. Insulin release was normalized for the total islet insulin content shown in C. B, protein content per islet, and D, insulin content corrected by the protein content per islet. Means ± S.E. are of 15–20 separate determinations from islets of 5 ATGL−/− and 10 ATGL+/+ mice in five separate experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus ATGL+/+ for the same incubation condition; unpaired two-tailed Student's t test.