Figure 4.
NO and 3-nitrotyrosine are increased in bleomycin-treated SP-D (−/−) mice. BAL and lung tissue from SP-D (−/−) and C57BL/6 WT mice was harvested 8 d after administration of 1 U/kg bleomycin sulfate. (A) Total bronchoalveolar lavage (BAL) NO was determined as previously published (28). Data were expressed as total BAL NO expressed in pmoles (X ± SE, n = 3–5 per group; *p < 0.05 versus WT sham control. #p < 0.05 versus bleomycin-treated WT group). (B) Relative 3-nitrotyrosine levels in lung homogenates were determined by solid phase ELISA as previously described (28, 44). Data were expressed as a percent of sham operated WT controls (*p < 0.05 versus corresponding sham group; #p < 0.05 versus bleomycin-treated WT group; X ± SE, n = 3–5 per group).