Figure 7.
A subpopulation of neurons are produced from EYFP-tagged PPEPs. EYFP+ (green) neurons in the P15 ventral cortex are stained with neuronal marker NeuN (A, red) and HuC/D (B, red). A, Orthogonal views of neuron at the crosshair, showing colocalization of EYFP with NeuN. The boxed area in B shows clustered EYFP+ neurons. C, EYFP+/NeuN+ neurons from P60 adult had the appearance of pyramidal neurons, and the cell marked with arrowhead is shown at higher magnification in C1 (EYFP, green; NeuN, red). The arrowheads and arrows in C1 point to apical (marked with asterisk) and basal dendrites, respectively, whereas the wavy arrows point to axon extending to subcortical whiter matter. D, PPEP-derived neurons (EYFP+/HuC/D+) are EdU (red) positive in the P15 ventral cortex. D1 depicts five consecutive 1 μm confocal images in the z-dimension of the neuron indicated by arrowhead in E, showing colocalization of EYFP, HuC/D, and EdU. Scale bars: A, D, 30 μm; B, C, 50 μm.