FIG. 7.
Viral particles pseudotyped with HIV envelope do not induce expression of activation markers or cell cycle entry in resting CD4+ T cells. (A) Mock-infected or HIVvector(HIV Env)-infected pure resting CD4+ T cells (at ∼1 to 3 SST per cell in activated CD4+ T cells) were stained for CD4 and the cell activation markers CD25, CD69, and HLA-DR at various time points postinoculation. The gates for each time point were set using a fluorescence-minus-one control corresponding to each time point, similar to the method used for the experiment shown in Fig. 1A. (B) Mock-infected or HIVvector(HIV Env)-infected pure resting CD4+ T cells (at ∼1 to 3 SST per cell in activated CD4+ T cells) were stained with pyronin Y and 7-AAD to monitor cell cycle progression at 0, 4, and 24 h postinfection. The gates described in the legend of Fig. 1B were applied to establish the cell cycle stage.