Table II.
Th17 cells generated in vitro with TGF-β fail to induce EAE
| Differentiation conditions | IFN-γa | IL-17a | IL-10a | EAE incidence |
| TGF-β + IL-1β + IL-23 + IL-6 | 2.1 | 24 | 4.8 | 0/5 |
| TGF-β + IL-1β + IL-23 + αIL-6 | 4.8 | 1.5 | 1.3 | 0/5 |
| TGF-β + IL-1β + αIL-23 + IL-6 | 2.5 | 17 | 3 | 0/5 |
| TGF-β + αIL-1β + IL-23 + IL-6 | 1.5 | 12 | 2.3 | 0/6 |
| TGF-β + αIL-1β + αIL-23 + IL-6 | 1.7 | 16 | 1.7 | 0/6 |
| TGF-β + αIL-1β + IL-23 + αIL-6 | 3.7 | 0.9 | 1.2 | 0/5 |
| TGF-β + IL-1β + αIL-23 + αIL-6 | 6.6 | 1.6 | 1.6 | 0/5 |
Bolded terms indicate antibodies to a specific cytokine.
a
The percentage of cytokine-positive CD4+ T cells as determined by intracellular flow cytometric analysis. ELISA was used to measure cytokine production and correlated with the percentage of cytokine-positive CD4+ T cells.