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. 2009 Jul 6;206(7):1549–1564. doi: 10.1084/jem.20082584

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© 2009 Yang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Encephalitogenicity correlates with IFN-γ production and T-bet expression. (A) Naive splenocytes from wild-type B10.PL mice were activated in vitro with anti-CD3/CD28 plus different combinations of cytokines for 72–96 h. ELISA was performed to detect IL-17 and IFN-γ secretion. Data are representative of three independent experiments (means ± SEM). (B) Naive Vα2.3/Vβ8.2 transgenic splenocytes were differentiated in vitro with MBP Ac1-11 plus different combinations of cytokines for 3 d and transferred into naive B10.PL mice. Data are representative of two independent of experiments (means ± SEM). (C) Cells from B were harvested and flow cytometry was used to evaluate IFN-γ, IL-17, IL-10, and T-bet (white, T-bet; gray, isotype control) expression in CD4⁺ T cells before transfer. Data are representative of two independent experiments (percentages are shown).