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MiR-155 targets PU.1 3′-UTR. Dual luciferase assay was performed on HeLa cells transfected with pGL3 (normalizing control) and pRLTK (Control), pRLTK_WT_3′UTR_PU1 (WT), or pRLTK_MUT_3′UTR_PU1 (Mut). These plasmids were co-transfected with pCDNA3.1 (Control) or pCDNA3.1-BIC (miR-155). Normalized Renilla luciferase values were represented relative to the control in each case. Data represent mean ± S.D. (error bars). *, statistically significant, ≤0.05.
