Figure 9.
Adjuvant mediated TIDC maturation does not influence Ag uptake. 50µg of EαGFP alone, or with CpG, polyI:C, or LPS (same volume) was injected either s.c. or i.t. CD11b+ DC in DLN were examined for GFP fluorescence and YAe antibody staining 24hr later (A). Numbers in the plots indicate percent cells in the top right quadrant. GFP MFI of GFP+CD11b+ DC in DLN samples was quantified (B). GFP MFI of GFP+ dermal DC or TIDC was quantified 4hr post injection (C). Percent fully mature DC (I-Ab+brightCD86+) 4hr post injection was quantified (D). GFP+ dermal DC and TIDC were examined for percent YAe+ cells 24hr post injection (E). Data are representative of two independent experiments.
