Figure 4.
TIDC are intrinsically defective in Ag uptake. 50µg or 500µg of EαGFP (same volume) was injected either s.c. into naive or i.t. into B16.F10-bearing animals. Dermal DC and TIDC were examined for GFP fluorescence and YAe antibody staining 3 and 4hr, respectively, post injection (A). Percent GFP + (B) and fold increase in GFP MFI from no injection control (C) for dermal DC and TIDC were quantified at indicated times post injection of 50µg of EαGFP. Dermal DC and TIDC were examined for GFP MFI 3 and 4hr, respectively, post injection of indicated quantities of EαGFP (D). DC were magnetically isolated and plated in vitro with indicated doses of EαGFP for 45 min at 37°C. Cells were then examined for intracellular GFP fluorescence (E). Tumor-free, B16.F10-bearing, EL4-bearing, and 4T1-bearing animals were injected with 50µg EαGFP. GFP MFI for dermal DC and TIDC was quantified 4hr after injection (F). Data are representative of at least two independent experiments.