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. 2009 May 11;77(7):2657–2671. doi: 10.1128/IAI.01523-08

TABLE 2.

Phenotypic characterization of KLA-attenuated mutantsa

Function and mutant strain Gene designation In vitro CIb
Uronic acid contentc
LB M9-glucose
Cell metabolism
    VA01 galK 1.037 ± 0.006 1.007 ± 0.038 18.9 ± 2.7
    VA04 proV 1 ± 0.017 0.993 ± 0.051 14.5 ± 1.8
    VA09 araF 1.017 ± 0.006 0.96 ± 0.02 14.2 ± 0.9
    VA17 rhaB 1.063 ± 0.012 0.683 ± 0.081 13.1 ± 1.5
    VA24 gabD 1.027 ± 0.025 0.93 ± 0.017 12.2 ± 1.0
    VA29 pgdH 0.987 ± 0.006 0.967 ± 0.032 21.9 ± 2.1
    VA32 galT 1 ± 0.01 0.947 ± 0.015 19.6 ± 2.7
    VA33 ahpC 1.017 ± 0.025 0.937 ± 0.059 18.5 ± 3.8
Cell surface components and transporters
    VA05 mrkC 1.007 ± 0.006 0.94 ± 0.064 12.5 ± 0.9
    VA08 hgpA 0.98 ± 0.015 0.95 ± 0.02 14.5 ± 1.4
    VA10 pls 1.02 ± 0.026 1.013 ± 0.049 23.2 ± 3.8
    VA13 pteA 0.983 ± 0.021 0.963 ± 0.025 13.3 ± 2.3
    VA22 pteC 0.987 ± 0.025 0.963 ± 0.042 19.5 ± 1.2
    VA23 fimC 1 ± 0.01 0.97 ± 0.026 24.5 ± 2.1
    VA27 pagO 0.967 ± 0.012 0.917 ± 0.015 20.0 ± 1.5
    VA28 uraA 0.997 ± 0.032 0.95 ± 0.03 12.5 ± 1.8
Regulation
    VA02 cbl 1.03 ± 0.02 1.027 ± 0.047 15.3 ± 0.9
    VA12 kvgA 1.017 ± 0.012 0.983 ± 0.05 13.8 ± 1.5
    VA14 moaR 1.03 ± 0.02 0.96 ± 0.046 13.4 ± 3.2
    VA18 kvgS 1.003 ± 0.012 0.933 ± 0.023 15.0 ± 2.0
    VA25 csgD 0.983 ± 0.021 0.957 ± 0.045 16.6 ± 2.0
Hypothetical and others
    VA07 Novel 0.997 ± 0.011 0.98 ± 0.026 15.0 ± 1.2
    VA11 yfgG 1.02 ± 0.017 0.983 ± 0.059 19.0 ± 4.4
    VA15 Novel 0.99 ± 0.017 0.97 ± 0.01 27.6 ± 7.3
    VA19 Novel 0.977 ± 0.021 0.967 ± 0.015 17.6 ± 2.1
    VA20 int 0.997 ± 0.035 0.937 ± 0.038 13.5 ± 1.0
    VA21 ymdF 0.963 ± 0.021 0.99 ± 0.01 19.7 ± 2.9
a

The HV phenotypes of the colonies were determined using the string-forming test with blood agar, as described previously (13). All strains showed positive results, defined as the formation of a ≥10-mm length of string, like that formed by the wild-type strain. Bacterial resistance to serum killing was determined as described previously (13). If the bacteria examined showed a reduction of 2 log10 in CFU after 30 min of incubation with nonimmune human serum at 37°C, the strain was defined as serum sensitive. If the viability of a VA mutant was comparable to that of the parental strain, the VA mutant was considered serum resistant. All strains were serum resistant.

b

CI values were determined as described previously (16) and are shown as means ± standard deviations.

c

The uronic acid content levels of the capsular polysaccharides were determined as described previously (24) and are expressed as means ± standard deviations (μg/1010 CFU). The value for the parental strain was 17.0 ± 2.2.