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. 2009 May 11;77(7):2657–2671. doi: 10.1128/IAI.01523-08

TABLE 1.

Genetic loci required for K. pneumoniae in the development of KLA

Function and mutant straina STM no. Gene designationb Similarityc
Function Predicted polarity
Gene % Identity (% similarity) Genus
Cell metabolism
    VA01 E0505 galK Klebsiella Galactokinase None
    VA04 E1013 kva04 proV 92 (96) Salmonella Glycine betain/l-proline ABC transporter None
    VA09 G1201 kva09 araF 91 (96) Escherichia l-Arabinose binding periplasmic protein None
    VA17 E2605 kva17 rhaB 41 (59) Lactobacillus α-l-Rhamnosidase, putative None
    VA24 E2945 kva24 gabD 91 (95) Escherichia Succinate-semialdehyde dehydrogenase gabT and gabP
    VA29 C5243 kva29 pgdH 34 (54) Serratia d-3-Phosphoglycerate dehydrogenase None
    VA32 E5721 galT Klebsiella Galactose-1-phosphate uridylyltransferase galK
    VA33 A5901 ahpC ahpC 99 (100) Salmonella Alkyl hydroperoxide reductase None
Cell surface components and transporters
    VA05 D1112 mrkC Klebsiella Usher protein of type III fimbriae mrkD and mrkF
    VA08 E1205 kva08 hgpA 28 (46) Haemophilus Hemoglobulin binding protein None
    VA10 G1706 kva10 pls 29 (33) Streptococcus Surface large repetitive protein None
    VA13 G1815 kva13 pteA 39 (60) Bacillus Cellobiose-specific phosphotransferase IIA None
    VA22 F2730 kva22 pteC 55 (75) Bacillus Cellobiose-specific phosphotransferase IIC None
    VA23 A2805 fimC Klebsiella Chaperon protein of type I fimbriae fimD
    VA27 E5021 pagO Klebsiella PhoPQ-activated integral membrane protein None
    VA28 E5145 kva28 uraA 93 (97) Escherichia Uracil permease None
Regulation
    VA02 F0514 cbl Klebsiella HTH-type transcriptional regulator Cbld None
    VA12 E1813 kvgA evgA 50 (66) Escherichia Response regulator of two component system kvgS
    VA14 F2014 moaR Klebsiella Monoamine regulon positive regulator None
    VA18 G2615 kvgS evgS 52 (68) Escherichia Histidine kinase sensor None
    VA25 E3045 kva25 csgD 39 (62) Salmonella Response regulator for second curli None
Hypothetical and others
    VA07 G1115 kva7 Hypothetical protein None
    VA11 D1812 kva11 yfgG 77 (91) Escherichia Hypothetical protein YfgG None
    VA15 E2145 kva15 Putative LuxR family transcription regulator None
    VA19 C2619 kva19 Putative UphA family transcription regulator None
    VA20 D2620 kva20 int 92 (96) Escherichia Putative integrase None
    VA21 C2635 kva21 ymdF 85 (94) Escherichia Conserved hypothetical protein YmdF None
    VA26 H4601 kva26 lyxK 31 (49) Pasteurella Putative l-xylulokinase pgdH
a

Twenty-eight KLA-attenuated mutant strains are shown and categorized by their putative functions. Class I mutant strains are indicated in bold.

b

Known K. pneumoniae genes or ORFs identified as kva (klebsiella virulence associated).

c

Genes encoding proteins or hypothetical proteins in the public database with the highest similarity to the sequences identified. Identities and similarities were determined at the amino acid level over the entire ORF.

d

HTH, helix-turn-helix DNA binding motif.