Fig. 2.
Generation and rescue of Orc6 mutant. Fragment of genomic map from Drosophila database and limits of the orc635 deletion are shown (A). Drosophila wild-type (DmOrc6), human (HsOrc6), truncated C terminus mutants (DmOrc6-220, DmOrc6-200), and substitution mutant (DmOrc6-WK228AA), all fused with GFP, were used in the rescue experiments (B). The predicted cytokinesis domain of Orc6 is shown in white. Conservative tryptophan (228) and lysine (229) amino acid residues of Drosophila Orc6 were replaced with alanines to create DmOrc6-WK228AA clone. An alignment of corresponding Orc6 sequences between different species is shown in the box.