Fig. 3.
Human GBM cell lines express Dicer-mediated miR-222 and -339. (A) Relative expression of miR-222 (Left) and miR-339 (Right) in 4 human glioma cell lines, HCT116 WT (+/+) cells and HCT116 Dicer (ex5 −/−) cells by TaqMan analyses. *, P < 0.05 for the relative miR expression in glioma cell lines compared with that in HCT116 Dicer (ex5 −/−) cells. (B) U87 cells were transfected with either a control RNA duplex or a RNA duplex targeting Dicer mRNA. Error bars indicate SD. *, P < 0.05 for the relative miR expression in Dicer siRNA-transfected U87 cells compared with that in control siRNA-transfected U87 cells. (A and B) The relative miR expression level for each cell line was normalized to the small nuclear RNA RNU43 level and calculated as the relative threshold cycle (CT) value to that of the HCT116 Dicer (ex5 −/−) cells. The results represent mean ± SD of triplicate samples.