Fig. 1.
Dicer (ex5−/−) cells are more sensitive to CTL-mediated cytolysis association with up-regulation of ICAM-1. (A) CTLs raised against IL-13Rα2345–353:1A9V (Left) or EphA2883–891 (Right) were tested for their lytic ability against human colorectal cancer HCT116 cells (solid square, HLA-A2+, EphA2+, IL-13Rα2+), HCT116 Dicer (ex5−/−) cells (hollow square, HLA-A2+, EphA2+, IL-13Rα2+), or T2 cells loaded with IL-13Rα2345–353:1A9V (solid circle on Left), EphA2883–891 (solid circle on Right) or Influenza M158–66 (hollow circle) using 4-hour 51Cr-release assays. Values indicate averages of triplicate samples. Bars indicate SD. (B) ICAM-1 was up-regulated in HCT116 Dicer (ex5−/−) cells. Flow cytometric analyses were performed on wild-type or Dicer (ex5−/−) HCT116 cells for expression of a panel of proteins that are known to mediate CTL-tumor cell interactions. Open and shaded histograms represent wild-type and Dicer (ex5−/−) HCT116 cells, respectively. Dashed lines represent control cells stained with isotype control IgG.