Figure 4.
Dissociation of the I1 dynein complex in bop5-2 dynein extracts. (A) Western blot of sucrose gradient fractions from a wild-type dynein extract probed with antibodies against several I1 dynein subunits. The I1 dynein subunits cosediment and peak at >20 S. FAP120 does not copurify with I1 dynein after salt extraction of wild-type axonemes (Ikeda et al., 2009) and thus was not analyzed here. (B) Western blot of sucrose gradient fractions from a bop5-2 dynein extract probed with antibodies against I1 dynein subunits. IC140 and the 1α DHC cosediment at >12 S, but the I1 dynein LCs Tctex1 and Tctex2b dissociate and sediment near the top of the sucrose gradient.