Figure 1.
Increased apoptosis and inflammation in leptin-deficient obese and lean sham mice. (A) Mean ± SEM per cent apoptosis (left panel) and ×20 power representative immunofluorescent staining of cardiac sections from sham mice (right three panels). Nuclei (stained with DAPI) are shown in blue, and all TUNEL-positive cells in the field are shown in red (left facing arrow), and accompanying scale bar (10 µM) in white. (B) As in (A), except that per cent CD45 cells (left panel) with representative images (right three panels, single example at left facing arrowhead) are shown in green. (C) As in (A), except that per cent TUNEL-positive/CD45-negative cells with representative images [right three panels, examples of TUNEL-positive/CD45-positive cell (in yellow; left facing arrow) and TUNEL-positive/CD45-negative cell (in red; right facing arrow)] are shown. *P < 0.05 vs. wild-type sham.