Figure 4.
NK1 receptors in the rostral VRC of the mouse. Pseudocolored sagittal section through the rhombencephalon of the mouse with immunolabeled NK1 receptors (cyan). Note the dense labeling at the level of the preBötC and also at the ventral and caudal portions of the facial nucleus. Nevertheless, in comparison to areas such as the superior olive (SO) and lateral reticular nucleus (LRt), which are essentially unstained for NK1 receptors, few areas of the VRC (i.e. the RTN, BötC, preBötC, or rVRG) can be considered devoid of NK1 receptors.
Following immunolabeling with diaminobenzidine as the chromogen, the section was subsequently counterstained with ethidium bromide (red) as a fluorescent Nissl stain. Grayscale images of the NK1 receptors were inverted with respect to black and white and copied to the green and blue channels of an RGB image. A grayscale image of the Nissl staining was copied to the red channel of the same image to produce the final composite image.