Figure 8.
NGA Activity Promotes Expression of Members of the STY Family.
(A) and (B) Normalized expression of six members of the STY gene family, including STY2 (A) and six members of the auxin-responsive GH3 genes (B) in wild-type inflorescences compared with lines lacking activities of the four NGA genes.
(C) to (H) Expression of STY2:GUS in wild-type ([C] to [E]), CRC>>NGA1 ([F] and [G]), and 35S:amiR-NGA164a (H) flowers. In the wild type, STY2:GUS is not expressed in the style of stage 9 gynoecia (C) (arrow) but becomes apparent in the style of stage 10 flowers ([D]; arrow). In stage 12-13 wild-type gynoecia (E), expression is confined to the style. In stage 9 CRC>>NGA1 flowers (F), expression can be observed at the top of the gynoecium and the nectary positions (arrows).
(G) By stage 13, expression is strong in the nectary positions and is basally expanded at the top of the gynoecium in CRC>>NGA1 flowers (arrows).
(H) A stage 12, 35S:amiR-NGA164a flower where STY2:GUS expression is restricted to low levels at the tips of the valve-like outgrowths (arrow).
Bars = 100 μm.