FIG. 4.
Phenotypic analyses of the stk1 mutant in S. aureus. (A) Purine auxotrophy of the stk1 deletion mutant strain in S. aureus strain 8325-4. Cell growth was monitored at OD600 in RPMI 1640 synthetic medium at 37°C with or without 200 μM adenine (Ad). ▪, wild-type (WT) strain (8325-4) without adenine; ♦, wild-type strain (8325-4) with 200 μM adenine; ▴, strain ST1004 (Δstk1) without adenine; ×, strain ST1004 (Δstk1) with 200 μM adenine. (B) Purine auxotrophy is corrected in the complemented strain. Cell growth was monitored at OD600 in RPMI 1640 synthetic medium. ▴, strain ST1010 (Δstk1 pMK4-Pprot); •, strain ST1008 (Δstk1 pC2). (C) Triton X-100-induced autolysis of S. aureus strains. Shown are strain 8325-4 without (□) or with (▪) 0.1% Triton X-100 and strain ST1004 (Δstk1) without (▵) or with (▴) 0.1% Triton X-100. (D) Triton X-100-induced autolysis in the complemented strain. ▴, strain ST1010 Δstk1 pMK4-Pprot; •, ST1008 Δstk1 pC2. (E) Sensitivity to fosfomycin. Cultures were grown in TSB medium at 37°C in the presence of fosfomycin (9 μg/ml). ▪, strain 8325-4; ▴, strain ST1004 (Δstk1).