Figure 4.
Effects of dmiro mutations on the time mitochondria allocate to plus and minus end- directed mitochondrial movements. A, B, Average percentage of time AM (A) and RM mitochondria (B) allocated to plus end-directed [(+)end] or minus end-directed [(−)end] trips. Genotypes: control, homozygous dmiro null mutants (Null −/−), homozygous dmiro null mutants during expression of normal dMiro protein (Rescue), and during dMiro overexpression (OE-10). Significant differences among all genotypes are indicated by asterisks (p < 0.05, N > 18, two-way ANOVA). Error bars represent SEM. C, D, Average percentage of time AM (C) and RM mitochondria (D) allocated to plus end-directed [(+)end] runs, minus end-directed [(−)end] runs, and short stationary phases (stops). Genotypes: as in A with the addition of heterozygous dmiro null mutants (Null −/+). Significant differences are indicated (p < 0.05, N > 18, two-way ANOVA). Error bars represent SEM. E, F, Average frequency of stops for AM (E) and RM mitochondria (F). Significant differences indicated (p < 0.05, N > 18, one-way ANOVA). Genotypes are as in A and B. Error bars represent SEM. G, H, Average frequency of reversals in direction for AM (G) and RM mitochondria (H). Significant differences are indicated (p < 0.05, N > 18, one-way ANOVA). Genotypes are as in A and B. Error bars represent SEM.