Figure 2.
Elevated glucose and T0901317 increase de novo lipid synthesis in INS-1 cells. INS-1 cells were cultured for 48 h in 4 or 16.7 mm glucose ± 10 μm T0901317. Cells were then incubated overnight under the same conditions in the presence of [2-14C]acetic acid. A, Total lipids were extracted from the cells and separated on silica TLC plates with hexane-ether-acetic acid (90:30:1). Plates were dried, and radioactivity was detected and quantified on a PhosphoImager (Molecular Dynamics). Data are reported as percentage of total labeled lipid. Percent of labeled polar lipid is reported as a numeric value at the top of the graph. B, Total lipids were saponified and incorporation of 14C into fatty acids was determined by reverse-phase HPLC. Data are reported as percentage of total labeled fatty acid. Values are the mean ± sem for four independent experiments.