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. 2009 May 11;106(21):8623–8628. doi: 10.1073/pnas.0809818106

Fig. 2.

Fig. 2.

Enhanced cytokine production by virus-specific CD8+ T cells in MHC I−/− BM chimeras. (A) IFN-γ and TNF-α production by GP33-, GP276-, and GP118-specific CD8+ T cells from spleens of chimeric mice 15 days after LCMV CL-13 infection. Numbers represent the percentage of IFN-γ+ CD8+ T cells that are TNF-α+. (B) The number of splenic CD8+ T cells producing IFN-γ upon stimulation with either of 6 LCMV peptides as indicated. (C) Mean fluorescence intensity (MFI) of IFN-γ expression in the 6 antigen-specific CD8+ T cell populations after CL-13 infection. (D) TNF-α production by IFN-γ+ CD8+ T cells upon stimulation with the indicated peptides. (E) The number of gp33- or gp276-specific CD8+ T cells from the liver or lung producing IFN-γ upon stimulation. (F) Equivalent GP61-specific CD4+ T cell responses in the spleen after infection, as measured by production of IFN-γ by antigen-specific cells. (G) Viral titers in the blood, spleen and liver in chimeric mice 15 days after LCMV CL-13 infection. Numbers above WT → MHC I−/− bars represent fold increase in viral titer over that in WT → WT mice. *, P = <0.05; **, P = <0.01; error bars represent SEM. One representative experiment of 2–3 is shown.