Figure 3.
The long 3′-UTR of PGA1 mRNA is sufficient to target a NMD-insensitive mRNA to NMD. The mRNA schematics and northern blot analysis of miniPGK1 (A), and miniPGK1-PGA1 3′-UTR (B) mRNAs. The mRNA half-lives (T1/2) were determined as described in Figure 2 and are shown to the right of the phosphorImages. They are an average of three independent experiments, with the exception of the half-life for the miniPGK1 mRNA in the upf1Δ strain (A), which is an average of two experiments. The miniPGK1 mRNA half-lives measured here are consistent with the half-lives determined for this mRNA by Hagan et al. (28).