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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2008 Dec 11;46(6):858–866. doi: 10.1016/j.yjmcc.2008.11.019

Figure 3. Signalosomes from treated hearts induce mitoKATP opening in mitochondria from untreated hearts.

Figure 3

Shown are changes in “MitoKATP activity (%)” when signalosome fractions from rat hearts treated with bradykinin, ouabain, ischemic preconditioning, or ischemic postconditioning were added to assay mitochondria from untreated rat hearts. Ischemic preconditioning consisted of two 5 min ischemia-reperfusion cycles, and ischemic postconditioning consisted of six 10 s cycles of ischemia-reperfusion. Also shown are the effects of PP2A (11 ng/mL) and KT5823 (“KT”, 0.5 µM) added to the assay. Note that PP2A cannot cross the MOM of intact mitochondria, so its effect is on the MOM. Data are averages ± SD of 3 to 4 independent experiments. The figure is from Quinlan, et al. [39].