FIG. 2.
UV-exposed Rev1B/B and Rev1−/− MEFs display similar reductions in tract lengths of DNA fibers. (A) Schematic representation of replication labeling and representative replication forks during ongoing and stalled replication. (B) Representative set of DNA fibers of wild-type (Wt), Rev1B/B, and Rev1−/− MEFs exposed to 0, 20, or 40 J/m2 UV-C. (C) Average ratio of IdU to BrdU in wild-type, Rev1B/B, and Rev1−/− MEFs and Rev1 mutant MEFs complemented with mouse Rev1. (D) Distribution of percentages of replication forks at corresponding IdU/BrdU ratios. Blue bars, 0 J/m2 UV-C; red bars, 20 J/m2 UV-C; green bars, 40 J/m2 UV-C. (E) Length of replicating forks during BrdU labeling in unexposed wild-type cells and wild-type, Rev1B/B, and Rev1−/− MEFs at 20, 40, and 60 min after exposure to 20 J/m2 UV-C.