Fig. 7.
Autoradiography of Angiotensin II receptor binding in longitudinal consecutive sections of the ligated sciatic nerve. (A) Autoradiogram showing AT1 receptor binding in intact sciatic nerve considered as a control; (B) Sections showing total binding in the presence of [125I]-Sar1-Ang II. Consecutive sections showing binding as in A with addition of: (C) PD123319 to displace binding to AT2 receptors; (D) losartan to displace binding to AT1 receptors; (E) unlabeled Ang II displacing binding to AT1 and AT2 receptors (see Methods). Binding in the ligated sciatic nerve was studied in 2 proximal (P1 and P2) and 2 distal (D1 and D2) regions at 1mm intervals. Arrows point to the proximal (P1) region of the sciatic nerve revealing a high accumulation of AT1 receptor binding. (F) Quantitative determination of Ang II receptor binding in proximal and distal regions of the ligated sciatic nerve. *p<0.05 vs. all other groups. Scale bar = 1 mm. NS, non specific.