FIGURE 4.
Sch9 is required for high levels of tRNA and RP gene transcription. The indicated strains were treated with rapamycin for 0, 60, or 90 min, and total RNA was prepared for Northern analysis of pre-tRNALeu (A) or RP mRNAs (RPL28 and RPL3) (B). The data were quantified, normalized for loading relative to U3 snRNA, and plotted relative to the amount of transcript before rapamycin addition. WT, wild type; mins Rap, minutes of rapamycin. Pre-tRNA data are in C, and RP mRNA data are in D. The panels in A are non-adjacent lanes from the same gel.