Table 1.
Mouse strain–prion strain combinations
| Mouse strain | Prnp genotype | Prion strain | End pointa (days/weeks) | Harvest intervalb (weeks) |
|---|---|---|---|---|
| C57BL/6J (B6) | a/a | RML | 161/23 | 2 |
| C57BL/6J (B6) | a/a | 301V | 287/41 | 4 |
| C57BL/6.I-1 (B6.I) | b/b | RML | 336/48 | 4 |
| C57BL/6.I-1 (B6.I) | b/b | 301V | 126/18 | 2 |
| FVB/NCr (FVB) | a/a | RML | 154/22 | 2 |
| FVB-Tg(PrP-A)4053 (Tg4053) | >30a | RML | 56/8 | 1 |
| (FVB x FVB.129-Prnptm1Zrch)F1 (0/+) | a/0 | RML | 392/56 | 4 |
| FVB.129-Prnptm1Zrch (0/0) | 0/0 | RML | 196/28c | 4 |
aEnd point is equivalent to the incubation time in this study, which is defined as the interval between prion inoculation to end point. With the exception of 0/0 mice, all animals were at the terminal stages of disease when killed for brain harvest. For each combination, a set of mice was inoculated with brain homogenate from normal mice and brains were harvested at the same time points as prion-infected mice.
bBrains from three mice were taken at each time point. In a few instances, the interval deviated from that shown. For example, the final interval for B6-RML was 1 week due to severity of illness in these mice. The actual intervals are indicated in figures that follow.
cAdditional three RML-inoculated and three normal brain homogenate-inoculated 0/0 mice were aged 357 days (51 weeks).