Fig. 2.
Lack of SRC-2 results in features like those of Von Gierke's disease (glycogen storage disorder–1a). (A) SRC-2 ablation results in fasting hypoglycemia. Blood glucose concentrations were determined in mice upon ad libitum feeding and after 24 hours of fasting (n = 5 mice per group), by means of a hand-held glucometer. (B) Insulin concentrations were determined in plasma isolated from SRC-2 null mice and WT littermates upon 24 hours of fasting (n = 5 mice per group). (C) SRC-2 ablation results in increased concentrations of triglycerides, cholesterol, FFAs, and ketone bodies in the plasma upon fasting. Plasma concentrations of triglycerides, cholesterol, FFAs, ketone bodies, uric acid, and lactic acid were determined from plasma isolated from SRC-2 null mice and WT littermates upon 24 hours of fasting. (D to G) SRC-2 ablation results in liver glycogen accumulation and liver steatosis upon fasting. Liver tissue was isolated from SRC-2 null mice and WT littermates upon 24 hours of fasting. Formalin-fixed liver sections were stained with hematoxylin and eosin. (D) and PAS (E) for glycogen. Frozen liver sections were stained with Oil Red O (F) to demonstrate neutral lipids. Sections fixed in 2% gluteraldehyde and viewed by EM (G) demonstrated the abundance of lipid. (H) Measurement of hepatic glycogen and triglyceride content in mice fasted 24 hours. Data are represented as the mean ± SEM. Unpaired student's t test was used for evaluation of statistical significance. *P < 0.05, **P < 0.01, and ***P < 0.001.