Fig. 8.
Relative expression change of signature proteins and mRNA in subnetwork 1, tumor versus normal, for three patients (507, 534, and 540). mRNA values are the difference between the means measured using the normal samples (n = 16) and stage D samples (n = 50) obtained from the microarray. Protein -fold change was determined by label-free mass spectrometry except for IGFBP3, which was determined by Western blot. As most targets were up-regulated in the tumor, carbonic anhydrase I (CA1) is included as a loading control to indicate that the observed up-regulation of most targets was not merely due to a greater amount of tumor digest on column versus normal.