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. Author manuscript; available in PMC: 2009 Apr 3.
Published in final edited form as: J Invest Dermatol. 2008 Oct 16;129(4):843–850. doi: 10.1038/jid.2008.320

Figure 3. Effects of LL-37 on the mRNA expression and production of COL1A1, COL1A2, and COL3A1 in human dermal fibroblasts.

Figure 3

(a) HDF cells were harvested after LL-37 (40 nm) and IFN-γ (1,000 U ml−1) treatment for 24 h. Total RNA was extracted, and cDNA was synthesized for real-time RT-PCR. Bars indicate mean±SD of three independent experiments performed, each with triplicate samples. (b) Cells were cultured in serum-free medium. Protein was determined in supernatants after 48 hours of culture. Total collagen was detected by sircol assay, each performed in triplicate. Data are mean±SD. *P<0.05 versus control.