FIG. 4.

Laboratory personnel basal serum 25(OH)D concentration. A, In these 10 individuals, the mean serum 25(OH)D varied widely [from 17.1 (4.6) to 35.6 (5.2) ng/ml; P < 0.005] between laboratories (error bars represent SEM). B, Similarly, marked within-individual variation was observed in 25(OH)D measurement in different laboratories. Whether an individual has hypovitaminosis D (arbitrary threshold, 32 ng/ml shown as dashed line) depends on which laboratory was used. Note that laboratory H used HPLC and was considered to be the “gold standard” against which others were compared. Reproduced with permission.33