FIG. 2.

C. jejuni induces an increase in levels of IL-6 expression. RNA extracted from Caco-2 cells treated for 3 h was analyzed by reverse transcription-PCR using primers specific to β-actin and IL-6. Products were compared against the GeneRuler 100-bp DNA ladder (Fermentas) using 2% agarose gel electrophoresis. Lactobacillus, L. rhamnosus GG; RT control, cells treated with PMA but with no reverse transcriptase added. PMA was used at 500 ng ml−1.