FIGURE 4.
The effect of miR-21 on H2O2-induced VSMC apoptosis and death. Cultured rat VSMCs pre-treated with vehicle, miR-21 inhibitor (LNA-anti-miR-21, 30 nm) or pre-miR-21 (30 nm) were treated with H2O2 (50 μm) for 24 h. Then, cell death was determined by trypan exclusion (A) and cell apoptosis was determined by TUNEL staining (B). Representative TUNEL-stained photomicrographs from VSMCs treated with vehicle (C), control oligonucleotide (D), Pre-miR-21 (E), and LNA-anti-miR-21 (F). Note: n = 6; *, p < 0.05 compared with vehicle control treated with H2O2 (50 μm).