Table 1. Primer and probe sequences.
| Primer/probe | Sequence (5′-3′) |
|---|---|
| F1 |
CGTTCTTCGGACGCCTCGTCAACAC |
| F2 |
GGATCCGTTCTTCGGACGCCTCGTC |
| A3 |
TCAGAAGCTATACGGCACAGCCAG |
| 108–03K-F |
GGACCATACAGGCCGAAGGAC |
| Neo-R1 |
CCTTCTATCGCCTTCTTGACGAGTTC |
| 182-R4 |
CCCAAGTCCTTTTCACCGAGAAGAG |
| miR182LNA |
tgTgaGttCtaCcaTtgCcaAa |
| miR182LNA/MM | tgTgaGttCtaCcaAtgCcaAa |
Primers used for genotyping the miR-182 KO mice are shown. The perfect-matched LNA-probe against miR-182 (miR182LNA) or the single-base mismatched control probe (miR182LNA/MM) was used for in situ hybridization. LNA positions are in capital letters, with DNA in lower case and the position of the single-base substitution in bold print.