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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Kidney Int. 2008 Oct 8;74(12):1526–1537. doi: 10.1038/ki.2008.500

Figure 3. Heterogeneity of proinflammatory cytokine production by CD11bhigh F4/80low and CD11bhigh F4/80high macrophages 24 h following kidney IRI.

Figure 3

Representative tracings from FACS analysis of expression of intracellular cytokines, including IL-1α, IL-6, IL-12p40/70, and TNF-α, in the (a) F4/80low and (b) F4/80high populations of macrophages in mouse kidney after 24 h of reperfusion (defined by gating on CD45+ CD11bhigh F4/80low and CD11blowF4/80high macrophages, respectively, as shown in Figure 2b) for sham operation (blue line), IRI (green line), or isotype control (red line). Max (%), percentage of F4/80low or F4/80high macrophages that are cytokine positive. Summary of proinflammatory cytokine production by (c) F4/80low macrophages and (d) F4/80high macrophages. Gating on the F4/80low population (c), the percentage of cells that produced IL-1α, IL-6, IL-12p40/70, and TNF-α increased significantly following IRI compared to sham (P < 0.05 for all IRI compared to sham groups). Values are mean ± s.e.; N = 3–6. Similarly, gating on the (d) F4/80high population, the percentage of cells that produced IL-1α, IL-6, IL-12p40/70 was unchanged following IRI compared to sham, but TNF-α increased significantly following IRI compared to sham (P < 0.05). Values are mean ± s.e.; N = 3–6.