Figure 3.
Effect of activated Notch-1 on cell cycle progression. A, thymidine synchronization. Transduced MDA-MB-231 cells were synchronized with 2 mmol/L thymidine and analyzed by Western blotting at the indicated time intervals after release. B, cell cycle analysis. Synchronized and transduced MDA-MB-231 cells were analyzed for DNA content by propidium iodide staining and flow cytometry at the indicated time intervals. The percentage of cells in each cell cycle phase is indicated. C, cell proliferation. Transduced MDA-MB-231 or MCF-7 cells were counted at the indicated time intervals, starting at day 1 (arrow). Columns, mean (n = 3); bars, SE. D, rescue from mitotic cell death. MDA-MB-231 cells were transduced with combinations of pAd-NIC, pAd-Control, and pAd-T34A; synchronized; and analyzed for DNA content by propidium iodide staining and flow cytometry. The percentage of cells in each cell cycle phase is indicated.