FIGURE 3.
Schematic of uORFs within select mammalian GADD34 5′UTRs. Representative cDNAs encoding GADD34-related sequences in GenBank™ are shown, including human (NM_014330), rat (NM_133546), hamster (L28147), and mouse (NM_008654) GADD34 5′UTRs. The AUG codon of the GADD34 ORF is shown. The length of the 5′UTR and the space between uORFs are indicated above (nt, nucleotides). Each box represents uORF1 and uORF2 and the size of the predicted translated uORF (aa, amino acid length). A close-up view of the overlap of the mouse GADD34 uORFs is shown below. The upstream uORF (uORF1) overlaps with the downstream uORF (uORF2) by 1 nucleotide. The stop codon of uORF1 is underlined, and the AUG of uORF2 is in gray.